pce mp53dd 41856 (Addgene inc)
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Addgene inc
pce mp53dd 41856
Pce Mp53dd 41856, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pce+mp53dd/pCE-mp53DD+(Plasmid+%2341856)/pm41850000-45-16-19
Average 95 stars, based on 80 article reviews
Pce Mp53dd 41856, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 80 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pce+mp53dd/pCE-mp53DD+(Plasmid+%2341856)/pm41850000-45-16-19
Average 95 stars, based on 80 article reviews
pce mp53dd 41856 - by Bioz Stars,
2026-09
95/100 stars
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Electroporation:Article Title: Genome-scale CRISPR screens identify PTGES3 as a direct modulator of androgen receptor function in advanced prostate cancer. Article Snippet: C42B cells were treated with 50 ng ml−1 nocodazole (Sigma) for 14 h before electroporation. .. Cas9–sgRNA RNP complexes were assembled with 100 pmol Cas9 protein and 130 pmol sgRNA before electroporation and combined with 375 pmol homology-directed recombination template and 1 μg Article Title: Genome-scale CRISPR screens identify PTGES3 as a direct modulator of androgen receptor function in advanced prostate cancer Article Snippet: C42B cells were treated with 50 ng ml −1 nocodazole (Sigma) for 14 h before electroporation. .. Cas9–sgRNA RNP complexes were assembled with 100 pmol Cas9 protein and 130 pmol sgRNA before electroporation and combined with 375 pmol homology-directed recombination template and 1 μg Knock-In:Article Title: Phase-separated NDF-FACT condensates facilitate transcription elongation on chromatin. Article Snippet: .. To generate the knock-in cell lines, 0.7 μg PX459-Cas9-gRNA, 0.2 μg Article Title: Phase-separated NDF−FACT condensates facilitate transcription elongation on chromatin Article Snippet: .. To generate the knock-in cell lines, 0.7 μg PX459-Cas9-gRNA, 0.2 μg Transfection:Article Title: Phase-separated NDF-FACT condensates facilitate transcription elongation on chromatin. Article Snippet: .. To generate the knock-in cell lines, 0.7 μg PX459-Cas9-gRNA, 0.2 μg Article Title: Phase-separated NDF−FACT condensates facilitate transcription elongation on chromatin Article Snippet: .. To generate the knock-in cell lines, 0.7 μg PX459-Cas9-gRNA, 0.2 μg Plasmid Preparation:Article Title: Integrative multi-omic analysis links TDP-43-driven splicing defects to cascading proteomic disruption of ALS/FTD pathways Article Snippet: Briefly, BJFF.6 iPSCs (RRID:CVCL_VU02) were pretreated with mTeSR (Stem Cell Technologies) supplemented with 1X RevitaCell (Thermo Fisher Scientific) for 1 hour. .. Then, approximately 1X10 cells were transiently co-transfected with 1.15ug of SM32.hTDP43.g9 plasmid (cloned into Addgene #43860; spacer below), 1.5ug of p3s-Cas9HC plasmid (Addgene #43945), 2.8ug of SM32.eGFP donor (sequence below), and 500ng of Article Title: Forward programming of human pluripotent stem cells to generate glutamatergic and GABAergic neurons in a tri-culture model with astrocytes. Article Snippet: .. After that, 500 ng of the donor plasmid and 100 ng of the Article Title: Ultrasound Control of Gene Expression in Human iPSCs via Heat Shock Promoters Article Snippet: The AAVS1‐CAG‐LoxP‐DsRed‐STOP‐LoxP‐GFP donor plasmid was a gift from the Xiaoping Bao lab. IMR90 hiPSCs at about 70% confluency in a 6‐well plate were detached by treatment with Accutase and incubating the well at 37°C for 10 min. .. Cells were pelleted was resuspended in 100 μL PBS (Gibco, #14190136, Waltham, MA, USA) containing 7 μg donor plasmid, 7 μg of espCas9‐T2gRNA, and 2 μg Article Title: Forward programming of human pluripotent stem cells to generate glutamatergic and GABAergic neurons in a tri-culture model with astrocytes Article Snippet: .. After that, 500 ng of the donor plasmid and 100 ng of the Sequencing:Article Title: Integrative multi-omic analysis links TDP-43-driven splicing defects to cascading proteomic disruption of ALS/FTD pathways Article Snippet: Briefly, BJFF.6 iPSCs (RRID:CVCL_VU02) were pretreated with mTeSR (Stem Cell Technologies) supplemented with 1X RevitaCell (Thermo Fisher Scientific) for 1 hour. .. Then, approximately 1X10 cells were transiently co-transfected with 1.15ug of SM32.hTDP43.g9 plasmid (cloned into Addgene #43860; spacer below), 1.5ug of p3s-Cas9HC plasmid (Addgene #43945), 2.8ug of SM32.eGFP donor (sequence below), and 500ng of |
